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KMID : 0545120020120040617
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 4 p.617 ~ p.621
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Optimization of SOD Biosynthesis by Controlling Sucrose Concentration in the Culture of Carrot Hairy Root
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Kim Ji-Hyeon
Yoo Young-Je
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Abstract
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In order to optimize the carrot hairy root culture for SOD production, a fed-batch culture of hairy roots was performed in a bioreactor. Maximum SOD activity was obtained when the hairy roots were transferred to the MS medium containing 110 g/l concentration of sucrose. By controlling the sucrose concentration (70 g/l sucrose for growth and 110 g/l sucrose for production, respectively) in a two-stage fed-batch culture, 29 g/l of the hairy roots was obtained based on the final dry mass. The volumetrically determined SOD activity and productivity in the fed-batch culture were about 6 times higher than those from the flask culture containing sucrose at 30 g/l concentration.
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